涔抽惖铔嬬櫧( Lactoferrin, LF)鏄竴绋潪琛�绱呯礌閻电祼(ji茅)鍚堣泲鐧�锛屽爆浜庤綁(zhu菐n)閻佃泲鐧藉鏃�銆備钩閻佃泲鐧藉ぇ閲忓瓨鍦ㄤ簬鍝轰钩鍕曠墿鐢熺墿楂旀恫涓紝鐢遍粡鑶滀笂鐨窗鑳炵敘(ch菐n)鐢�锛屼篃鏄汉楂旈珨娑插厤鐤噸瑕佺殑绲勬垚閮ㄥ垎锛岄�ｆ帴鍏堝ぉ鍜岄仼鎳�(y墨ng)鎬у厤鐤姛鑳�銆�
涔抽惖铔嬬櫧鐨勪繚璀蜂綔鐢ㄦ湁鎶楃檶銆佹姉鐐庡拰鍏嶇柅瑾�(di脿o)绡�(ji茅)娲绘�у拰鎶楄弻锛岄�欎簺寤ｆ硾鐨勪繚璀蜂綔鐢ㄦ槸閫氶亷鍚勭ó鐢熺墿浣滅敤姗熷埗瀵︾従(xi脿n)鐨�锛屼緥濡備钩閻佃泲鐧界祼(ji茅)鍚堥惖鐨勮兘鍔涳紝閭勬湁涔抽惖铔嬬櫧鑸囧垎瀛愬拰绱拌優绲勫垎鐨勭浉浜掍綔鐢�,浣垮緱涔抽惖铔嬬櫧鍏锋湁澶氱ó鐛ㄧ壒鐨勬姉寰敓鐗╂椿鎬�銆�
闅ㄨ憲鐢熷懡绉戝(xu茅)鐨勪笉鏂风櫦(f膩)灞曪紝涔抽惖铔嬬櫧鐨勬姉鑿屾鍒惰寤ｆ硾鍦伴亱鐢ㄥ埌鐢熺墿鍒惰棩銆佺編瀹圭編濡濄�佷繚鍋ラ鍝�銆侀啱(y墨)鐧傞啱(y墨)缇庣瓑鍚勫�嬬敘(ch菐n)妤�(y猫)銆傛湰娆″皬绶ㄥ皣鍖附澶氱瘒鏂囩嵒璀夋摎(j霉)鐐哄ぇ瀹剁洡榛炰钩閻佃泲鐧芥姉鑿屾鍒�銆�
1.涔抽惖铔嬬櫧鐨勭祼(ji茅)妲�(g貌u)
涔抽惖铔嬬櫧鏄竴鍊嬬敱绱�700鍊嬫皑鍩洪吀绲勬垚鐨�80kDa绯栧熀鍖栬泲鐧斤紝鍦ㄧ墿绋枔鍏锋湁楂樺害鍚屾簮鎬�銆備钩閻佃泲鐧芥搧鏈夊叐鍊嬪皪绋辩殑鍚屾簮鐞冪媭鍩燂細N-鐠�(hu谩n)鍜孋-鐠�(hu谩n);N-鐠�(hu谩n)灏嶆噳(y墨ng)浜庤偨閺堜腑鐨勭1-333鍊嬫皑鍩洪吀娈樺熀锛岃�孋-鐠�(hu谩n)鑸囩345-692鍊嬫皑鍩洪吀娈樺熀灏嶆噳(y墨ng)锛岄�欏叐鍊嬪煙鐨勫叐绔敱涓�娈电煭閺�α-铻烘棆鐩搁�ｆ帴銆傛瘡鍊嬬挵(hu谩n)鏈夊叐鍊嬪瓙鍩�(浜炲煙)锛歂1銆丯2鍜孋1銆丆2 绲勬垚锛屽苟鍒嗗垾鍖呭惈涓�鍊嬮惖绲�(ji茅)鍚堜綅榛炲拰涓�鍊嬬硸鍩哄寲浣嶉粸 [1]銆�
涔抽惖铔嬬櫧涓夌董绲�(ji茅)妲�(g貌u)[2]
涔抽惖铔嬬櫧鑳界祼(ji茅)鍚堝绋噾灞洟瀛愶細Fe2+銆丗e3+銆丆u2+銆乑n2+鍜孧n2+绛�锛屽畠涓昏浠ュ叐绋舰寮忓瓨鍦細瀵屽惈閻靛舰寮忕殑holo-lactoferrin鍜屼笉鍚惖褰㈠紡鐨刟po-lactoferrin銆傚叐鑰呮湁涓嶅悓鐨勪笁绱氱祼(ji茅)妲�(g貌u)锛歛po-lactoferrin鐨勭壒榛炴槸鏈変竴鍊�“闁嬫斁寮�”鐨凬-鐠�(hu谩n)鍜屼竴鍊�“灏侀枆寮�”鐨凜-鐠�(hu谩n)锛岃�宧olo-lactoferrin鍏╁�嬬挵(hu谩n)閮芥槸“灏侀枆寮�”鐨�[3]銆�
姝ｆ槸鐢变簬涔抽惖铔嬬櫧鍙互鍙�嗙祼(ji茅)鍚堥惖闆㈠瓙鐨勭壒鎬э紝濂犲畾浜嗗叾鎶楄弻浣滅敤姗熷埗鐨勫熀绀�(ch菙)銆�
2.涔抽惖铔嬬櫧鎶楃窗鑿屾鍒�
澶ч噺鐮旂┒鑰呯爺绌朵簡涔抽惖铔嬬櫧灏嶄簬闈╄槶姘忛櫧鎬ц弻(G+)鍜岄潻铇皬闄版�ц弻(G-)鍦ㄩ珨鍏�(n猫i)鍜岄珨澶栫殑鎶戣弻鎬ц兘鍜屾鍒讹紝绨″柈鍖附?c猫)缂︽嫝?/p>
琛�1 涔抽惖铔嬬櫧灏嶅父瑕嬭弻鏍殑鎶戣弻姗熷埗[2]
涔抽惖铔嬬櫧鐧�(f膩)鎻姉鑿屾椿鎬х殑浣滅敤姗熷埗鍙互姒傛嫭濡備笅锛�
2.1 閻甸殧闆㈡垨閻靛墲濂綔鐢細
閻甸洟瀛愭槸澶у鏁�(sh霉)绱拌弻鐢熼暦鎵�蹇呴渶鐨勭嚐椁�(y菐ng)鐗╄唱(zh矛)锛屽弮鑸囦簡瑷卞閲嶈鐨勭敓鐗╁(xu茅)閬庣▼銆備钩閻佃泲鐧藉叿鏈夎純楂樼殑閻甸洟瀛愯Κ鍜屾��锛屽彲浠ラ�氶亷铻悎閻甸洟瀛愯垏闇�瑕侀惖闆㈠瓙鐨勭窗鑿岀鐖�х祼(ji茅)鍚堥惖锛屽皣閻甸洟瀛愬緸绱拌弻鐥呭師楂斾腑闅旈洟鍑轰締锛岄檺鍒剁窗鑿屽湪鎰熸煋閮ㄤ綅灏嶉�欑ó鐕熼(y菐ng)鐗╄唱(zh矛)鐨勪娇鐢�锛屾姂鍒剁窗鑿岀殑鐢熼暦锛屽苟涓嬭(di脿o)鍏舵瘨鍔涘洜瀛愮殑琛ㄩ仈銆�
2.2 鏀硅畩绱拌弻绱拌優鑶滅殑閫氶�忔�э細
涔抽惖铔嬬櫧鑳藉鑸囩窗鑿岃〃闈㈠垎瀛愰潪鐗圭暟鎬х祼(ji茅)鍚堬紝灏侀枆绱拌弻绱拌優鑶滀笂鐨勯�氶亾浣嶉粸锛岄樆鏂风嚐椁�(y菐ng)鐗╄唱(zh矛)鍜岀窗鑿岃泲鐧界殑閬嬮��锛屽緸鑰屾姂鍒剁窗鑿岀殑鍚堟垚銆佷唬璎�銆傝剛澶氱硸(LPS)鐐洪潻铇皬闄版�ц弻绱拌優澶栬啘鐨勪富瑕佹垚鍒�锛屼钩閻佃泲鐧介�氶亷鑸囪剛澶氱硸鐨勭浉浜掍綔鐢ㄧ牬澹為潻铇皬闄版�ц弻鐨勫鑶�锛屾敼璁婄窗鑳炶啘鐨勯�氶�忔��[29]锛岄仈鍒扮洿鎺ユ鑿屼綔鐢�;涔抽惖铔嬬櫧N-绔付姝ｉ浕鑽蜂篃鍙互閫氶亷闃绘LPS鑸囩窗鑿岄櫧闆㈠瓙(Ca2+鍜孧g2+)涔嬮枔鐨勭浉浜掍綔鐢紝鏈夋晥骞叉摼澶ц吀妗胯弻鐨勮仛闆嗘�у娈�[30]銆�
2.3 鎶戝埗绱拌弻绮橀檮鎴栧畾妞嶏細
涔抽惖铔嬬櫧鐨凬-鏈鍏锋湁绲叉皑閰歌泲鐧介叾娲绘��锛岃兘鏈夋晥鍦板緸娴佹劅鍡滆妗胯弻(G-)澶栬啘涓彁鍙朓gA1铔嬬櫧閰跺墠铔嬬櫧锛岄倓鑳界壒鐣版�у湴闄嶈ВHap榛忛檮绱犲苟闃绘柗Hap浠嬪皫(d菐o)鐨勯粡闄�锛屽緸鑰岄槻姝㈡祦鎰熷棞琛�妗胯弻鐨勫畾妞�[31]銆備钩閻佃泲鐧藉叿鏈夐樆姝㈡煇浜涚窗鑿岄檮钁楀湪瀹夸富绱拌優涓婄殑鑳藉姏锛岀矘闄勬姂鍒剁殑姗熷埗灏氫笉娓呮锛屼絾鏈夌爺绌惰獚鐐�锛屼钩閻佃泲鐧界殑瀵＄敇闇茶仛绯栫祼(ji茅)鍚堢窗鑿岀矘闄勭礌锛岄樆姝㈠畠鍊戣垏瀹夸富绱拌優鍙楅珨鐨勭浉浜掍綔鐢�銆�
2.4 闃叉绱拌弻鐢熺墿鑶滃舰鎴愶細
鐢熺墿鑶滃舰鎴愭槸閵呯稜鍋囧柈鑳炶弻鐨勪竴绋兢鑱氱祫绻旂矘闄勬柟娉曪紝鍦ㄥ泭鎬х簴缍寲鎮ｈ�呬腑寰楀埌浜嗗厖鍒嗙殑鐮旂┒銆傞�氶亷鐢熺墿鑶滅殑褰㈡垚锛岀窗鑿屽皪瀹夸富绱拌優鐨勯槻寰℃鍒跺拰鎶楃敓绱犵殑娌荤檪鐢�(ch菐n)鐢熼珮搴﹁�愯棩鎬�锛岃�屼竴浜涚窗鑿岃弻鏍渶瑕侀珮姘村钩鐨勯惖渚嗗舰鎴愮敓鐗╄啘锛屾帹娓钩閻佃泲鐧介�氶亷閻佃灟鍚堜締鏈夋晥鎶戝埗鐢熺墿鑶滅殑褰㈡垚銆�
绺介珨鑰岃█锛屼钩閻佃泲鐧芥槸浜洪珨鍏�(n猫i)鍏锋湁鎶楀绋井鐢熺墿娲绘�х殑閲嶈鐗╄唱(zh矛)锛屾槸妲�(g貌u)鎴愪汉楂旈珨娑插厤鐤殑闂�(gu膩n)閸电祫鎴愰儴鍒�锛屽悓鏅備篃鍙互浣滅偤鐢熺墿鍒跺姂鐢熺敘(ch菐n)涓殑鐢熺墿闃茶厫鍔�锛岄啱(y墨)缇庤棩鍝佷腑鎶戣弻銆佹姉鑿屾垚鍒嗙殑閲嶈鍘熸枡锛屽叿鏈夊緢楂樼殑缍�(j墨ng)婵熷児鍊笺�傜洰鍓嶅競闈笂鐨勪钩閻佃泲鐧戒富瑕佸緸鐗涘ザ涓彁鍙�锛岀鍏冪敓鐗╀緷鎵樿嚜宸辩殑灏堝埄鎶�琛�(sh霉)骞宠嚭—— 姘寸ɑ鑳氫钩绱拌優鐢熺墿鍙嶆噳(y墨ng)鍣ㄩ珮鏁堥噸绲勮泲鐧借唱(zh矛)琛ㄩ仈骞宠嚭(OryzHiExp)鍜岄噸绲勮泲鐧界磾鍖栨妧琛�(sh霉)骞宠嚭(OryzPur)闁嬬櫦(f膩)浜嗘鐗╂簮閲嶇祫浜轰钩閻佃泲鐧斤紝鍏锋湁鑹ソ鐨勭敓鐗╂椿鎬�銆傛鐗╂簮閲嶇祫涔抽惖铔嬬櫧鏉滅禃浜嗗ぉ鐒朵钩閻佃泲鐧戒腑鍕曠墿婧�銆佷汉婧愰洔璩�(zh矛)鐨勯ⅷ(f膿ng)闅紝涔熸瘮寰敓鐗╂簮閲嶇祫铔嬬櫧鎿佹湁鏇村劒(y艒u)绉�鐨勬姌鐤婄媭鎱�(t脿i)锛岀祼(ji茅)妲�(g貌u)鍔熻兘鑸囩敓鐗╂椿鎬э紝鐐轰钩閻佃泲鐧藉競鍫存彁渚涗簡鏇村畨鍏ㄦ洿楂樿唱(zh矛)鐨勯伕鎿�銆�
鍙冭�冩枃鐛�
[1]Lactoferrin three-dimensional structure: a framework for interpreting function. 2000
[2]Lactoferrin a multiple bioactive protein: an overview.2012
[3]Lactoferrin: a multifunctional protein. 2005
[4]Effect of food components on growth of Basillius stearothermophilus. 1974
[5]Residues in colostrum following antibiotic dry cow therapy. 1984
[6]Inhibition of bacteria by lactoferrin and other iron-chelating agents. 1968
[7]Human milk lactoferrin inactivates two putative colonization factors expressed by Haemophilus inflfluenzae. 1998
[8]Potential antimicrobial effects of human lactoferrin against oral infection with Listeria monocytogenes in mice. 2005
[9]Identification of pneumococcal surface protein A as a lactoferrin-binding protein of Streptococcus pneumoniae. 1999
[10]Both lactoferrin and iron influence aggregation and biofilm formation in Streptococcus mutans . 2004
[11]Ultrastructural effects of antimicrobial peptides from bovine lactoferrin on the membranes of Candida albicans and Escherichia coli. 2005
[12]Antibacterial activity of lactoferrin and lactoferricin against oral Streptococci, Rom. 2010
[13]Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages. 2007
[14]Lactoferrin impairs type III secretory system function in enteropathogenic Escherichia coli. 2003
[15]Human milk fractions inhibit the adherence of diffusely adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) to HeLa cells. 2000
[16]Lichtenberger, Recombinant human lactoferrin is effective in the treatment of Helicobacter felis-infected mice. 2000
[17]Inhibition of Helicobacter pylori infection by bovine milk glycoconjugates in a BAlb/cA mouse model. 2001
[18]Iron acquisition by Helicobacter pylori: importance of human lactoferrin. 1993
[19]Metal complexes of lactoferrin and their effect on the intracellular multiplication of Legionella pneumophila. 2000
[20]Iron availability influences aggregation, biofilm, adhesion and invasion of Pseudomonas aeruginosa and Burkholderia cenocepacia. 2005
[21]Anti-bacterial activity of bovine milk lactoferrin against some mastitis causative pathogens with special regard to mycoplasma. 2010
[22]Loss of microbicidal activity and increased formation of biofilm due to decreased lactoferrin activity in patients with fibrosis cystic. 2004
[23]In vitro adhesion and invasion inhibition of Shigella dysentariae, Shigella flexneri and Shigella sonnei clinical strains by human milk proteins. 2004
[24]Human milk lactoferrin is a serine protease that cleaves Haemophilus surface proteins at arginine-rich sites. 2003
[25]Lactoferrin augments BCG vaccine efficacy to generate T helper response and subsequent protection against challenge with virulent Mycobacterium tuberculosis. 2005
[26]Anti-bacterial activity of bovine milk lactoferrin against some mastitis causative pathogens with special regard to mycoplasma. 2010
[27]Periodontitis, periodontopathic bacteria and lacto-ferrin. 2010
[28]Antimicrobial effect of lactoferrin and its amidated and pepsin-digested derivatives against Salmonella enteriditids and Pseudomona fluorescence. 2010
[29]Farnaud S, Evans RW. Lactoferrin--a multifunctional protein with antimicrobial properties. Mol. Immunol.. 2003,
[30]Damage of the membrane of enteric Gramnegative bacteria by lactoferrin and transferrin, Infect. Immun. 56 (1988) 2774–2781.
[31]Human milk lactoferrin inactivates two putative colonization factors expressed by Haemophilus influenzae. 1998
姝︽饥绂惧厓鐢熺墿绉戞妧鑲′唤鏈夐檺鍏徃
闆昏┍: 鍏徃绺芥锛�027-59403931 鐕熼姺閬嬬嚐涓績 18717109083
鍌崇湡锛�027-59301898
閮电锛� [email protected]
鍦板潃锛氭婕㈡澅婀栨柊鎶�琛�(sh霉)闁嬬櫦(f膩)鍗�(q奴)绁炲ⅸ浜旇矾268铏� 430070
