鍦ㄨ嚜鐒剁晫涓ぇ姒傚瓨鍦ㄨ憲9钀�(w脿n)澶氱ó鐪熻弻锛屽叾涓皪(du矛)浜洪鑷寸梾鐨勬湁500澶氱ó銆傜暥(d膩ng)浜哄嚭鐝�(xi脿n)姗�(j墨)楂旀姷鎶楀姏涓嬮檷銆佺毊鑶氱牬鎼�锛岄暦(zh菐ng)鏈熶娇鐢ㄥ唬璀滄姉鐢熺礌銆佹帴瑙稿埌鐪熻弻绛夋儏娉佹檪(sh铆)瀹规槗琚湡鑿屾劅鏌�銆傝嚚搴婇啱(y墨)瀛�(xu茅)鏍规摎(j霉)鐪熻弻鎰熸煋浜洪珨鎰熸煋鐨啔銆佹瘺鐧�(f膩)鍜屾寚鐢�锛屽父寮曡捣闋櫖銆佹墜鐧�佺毊鐧瓑锛屾繁閮ㄧ湡鑿屼富瑕佹劅鏌撶毊涓嬬祫绻�銆侀粡鑶滃拰鍏�(n猫i)鑷燂紝鍙紩璧风値鐧�銆佹桨鐦�銆佸姝荤瓑銆傚洜姝ょ櫦(f膩)鐝�(xi脿n)鍏锋湁鏈夋晥鎶楃湡鑿屾椿鎬х殑鐗╄唱(zh矛)闈炲父閲嶈锛屽凡鏈夌爺绌跺牨(b脿o)閬撻’绀猴紝涔抽惖铔嬬櫧鍏峰倷澶氱ó鎶楃湡鑿屽姛鑳�锛屽寘鎷皪(du矛)閰垫瘝鍜岄湁鑿岀瓑鐪熻弻鍏锋湁寤ｈ瓬鎶戝埗娲绘��锛岄倓鑳借垏鍏朵粬鎶楃湡鑿岃棩鐗╁崝(xi茅)鍚屾不鐧傜湡鑿屾劅鏌撳紩璧风殑鐥呯櫏銆�
鍙互瑾�(shu艒)涔抽惖铔嬬櫧鏄汉鍙婂嫊(d貌ng)鐗╅珨鍏�(n猫i)澶╃劧瀛樺湪鐨勬姉鐪熻弻琛�(w猫i)澹�锛�
涓婄瘒瑭崇窗(x矛)浠嬬垂浜嗕钩閻佃泲鐧界殑鎶楃窗(x矛)鑿屾(j墨)鍒讹紙榛�(di菐n)鎿婇�欓噷鍥為¨锛氫钩閻佃泲鐧芥姉鑿屾(j墨)鍒�锛�锛屾湰鏈熷氨渚�(l谩i)閲嶉粸(di菐n)鐩ら粸(di菐n)涓�涓嬩钩閻佃泲鐧芥姉鐪熻弻鐨勫姛鑳姐��
涔抽惖铔嬬櫧鏈夊叐绋舰寮忥細瀵屽惈閻靛舰寮忕殑holo-lactoferrin鍜屼笉鍚惖褰㈠紡鐨刟po-lactoferrin锛宧olo-lactoferrin闄ゅ湪涓夌礆(j铆)绲�(ji茅)妲�(g貌u)涓婅垏apo-lactoferrin涓嶅悓澶栵紝姣攁po-lactoferrin鏇磋�愯泲鐧芥按瑙1]銆傞櫎浜嗙祼(ji茅)鍚團e3+锛屼钩閻佃泲鐧介倓鍙互绲�(ji茅)鍚堜竴绯诲垪鍏朵粬鍖栧悎鐗╋紝濡傦細鑴傚绯�銆佽倽绱�銆丏NA鍜岄噾灞洟瀛愶紝鍖呮嫭Cu2+銆乑n2+鍜孧n2+ 绛�[2]銆備钩閻佃泲鐧藉皪(du矛)鍖呮嫭閰垫瘝鍜岄湁鑿屽湪鍏�(n猫i)鐨勪竴绯诲垪浜洪鍜屾鐗╃湡鑿岀梾鍘熼珨鍏锋湁鑹ソ鐨勬姂鍒舵椿鎬�(琛�1)銆�
琛�1锛氫钩閻佃泲鐧藉皪(du矛)閮ㄥ垎鐪熻弻鐨勬姂鑿岃兘鍔�
|
鐪熻弻椤炲瀷 |
MIC/IC50 |
鍙冭�冩枃鐛�(xi脿n) |
|
鐧借壊蹇电彔鑿� |
MIC=200--6400 ug/mL |
[3]銆�[4]銆�[5] |
|
鍏夋粦蹇电彔鑿� |
MIC≥6000 μg/mL |
|
|
鍏嬫煍蹇电彔鑿� |
IC50=20 μg/mL |
|
|
閲�閰掗叺姣� |
MIC=16 μg/mL |
|
|
鏍肩壒闅辩悆鑿�/鏂�鐢�闅辩悆鑿� |
MIC=64 μg/mL |
[6] |
|
榛戞洸闇� |
IC50= 578 μg/mL |
[7] |
|
缍犺壊鏈ㄩ湁 |
IC50=952μg/mL |
涔抽惖铔嬬櫧鐧�(f膩)鎻姉鐪熻弻娲绘�х殑浣滅敤姗�(j墨)鍒跺彲浠ユ鎷涓�:
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姗�(j墨)鍒朵簩锛氫钩閻佃泲鐧借垏鐪熻弻绱�(x矛)鑳炶〃闈㈢殑鐩存帴鐩镐簰浣滅敤锛屽皫(d菐o)鑷寸窗(x矛)鑳炶啘鎼嶅偡鍜屾徊婕忋��
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[7]Fungistatic activity of iron-free bovin lactoferrin against several fungal plant pathogens and antagonists.2008
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[9]Effect of lactoferrin and iron on the growth of human pathogenic Candida species. 2009
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[15] Ultrastructural effects of antimicrobial peptides from bovine lactoferrin on the membranes of Candida albicans and Escherichia coli. 2005
[16]Modulation of in vitro fungicidal activity of human lactoferrin against Candida albicans by extracellular cation concentration and target cell metabolic activity. 2004
[17]Human lactoferrin triggers a mitochondrial- and caspase-dependent regulated cell death in Saccharomyces cerevisiae. 2016
[18]Activated lactoferrin and fluconazole synergism against Candida albcians and Candida glabrata vaginal isolates. 2004
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